Genetska raznolikost planinskog bora (Pinus mugo Turra) u Hrvatskoj procijenjena pomoću morfometrijskih i molekularnih metoda

master's thesis

• Title (english): Genetic diversity of mountain pine (Pinus mugo Turra) in Croatia estimated with morphometric and molecular methods
• Author: Luka Benić
• Mentor: Ida Katičić Bogdan (mentor)
• Committee member: Ida Katičić Bogdan (predsjednik povjerenstva)
• Committee member: Igor Poljak (član povjerenstva)
• Committee member: Marko Bačurin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Forestry and Wood Technology; (Institute of Forest Genetics, Dendrology and Botany); Zagreb
• Defense date and country: 2024-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Forestry

Abstract

U ovom radu ispitana je morfološka, anatomska i genetska varijabilnost tri prirodne populacije planinskog bora u Hrvatskoj. Ukupno je analizirano sedam anatomsko-morfoloških značajki iglica i pet morfoloških značajki češera, dok je genetska raznolikost ispitana na temelju pet jezgrinih mikrosatelitnih biljega. Koeficijenti varijabilnosti na razini svih populacija zajedno kretali su se u rasponu od 8,71 % za debljinu iglica do 33,98 % za dužinu rukavca. Unutarpopulacijska varijabilnost bila je veća od međupopulacijske varijabilnosti za sve istraživane značajke. Statistički značajne pozitivne korelacije utvrđene su između mjerenih značajki iglica i češera. Multivarijatnim statističkim metodama utvrđeno je da se populacije planinskog bora u Hrvatskoj puno bolje mogu razlikovati na temelju anatomsko-morfoloških značajki iglica u odnosu na morfološke značajke češera. Općenito gledano, populacije s nižih nadmorskih visina u odnosu na one s viših imale su duže iglice, tanju epidermu i manji broj smolnih kanala. Molekularnimbiljezimaustanovljenajesrednjaočekivanaheterozigotnost od 0,54. Metode genetske diferencijacije razdvojile su populacije na statistički značajnoj razini. Trend grupiranja populacija bio je sličan kada se usporede multivarijatne analize u kojima su korištene morfološke značajke češera i mikrosatelitni biljezi. Općenito gledano, rezultati ovog istraživanja mogu poslužiti kao temelj za buduća istraživanja i programe očuvanja ove zanimljive, ali gubitkom staništa sve ugroženije planinske vrste.

Abstract (english)

In this study, the morphological, anatomical, and genetic variability of three natural populations of mountain pine in Croatia was examined. A total of seven anatomical-morphological characteristics of needles and five morphological traits of cones were analyzed, while genetic diversity was examined based on five nuclear microsatellite markers. The coefficients of variability at the level of all populations combined ranged from 8.71% for needle thickness to 33.98% for sheath length. Intra-population variability wasgreater than inter-population variability for all traits studied. Statistically significant positive correlations were found between the measured needle and cone traits. Multivariate statistical methods revealed that mountain pine populations in Croatia can be much better distinguished based on the anatomical-morphological needle traits compared to morphological cone traits. In general, populations at lower altitudes, compared to those at higher altitudes, had longer needles, thinner epidermis, and fewer resin canals. Molecular markers established an average expected heterozygosity of 0.54. Genetic differentiation methods separated the populations at a statistically significant level. The pattern of population grouping was consistent when comparing multivariate analyses based on morphological cone traits and microsatellite markers. Overall, the results of this study can serve as a basis for future research and conservation programs for this interesting but increasingly endangered mountain species due to habitat loss.

• Keywords: planinski bor; populacijska varijabilnost; morfometrijska analiza; anatomija iglica; morfologija češera; mikrosatelitni biljezi
• Keywords (english): mountain pine; population variability; morphometric analysis; needle anatomy; cone morphology; microsatellite markers
• Language: croatian
• URN:NBN: urn:nbn:hr:108:850380
• Study programme: Title: Graduate Studies Silviculture and Forest Management with Wildlife Management; specializations in: Graduate Studies - Programme: Silviculture and Forest Management with Wildlife Management; Course: Graduate Studies - Programme: Silviculture and Forest Management with Wildlife Management; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra inženjer/inženjerka šumarstva (sveučilišni/a magistar/magistra inženjer/inženjerka šumarstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-11 10:33:51